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The prepared samples of the solution and hydrogels were designated as #1, #2, #3, and #4.
Progress of the dissolution reaction was monitored by taking samples of the solution (5.0 ± 0.01 mL) at different times established according to the total reaction time (tr) of each experiment (≈20 samples per experiment).
Samples of the solution were intermittently analyzed for PO2, PCO2 and pH.
Thus, samples of the solution (60 μl) were taken every 2 min and the urea content was determined by diacetylmonoxime and thiosemicarbazide colorimetric assay (modified from Killingsbaeck [ 28]).
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The randomly-extracted samples are further adjusted with scaled-chaotic sequences to provide more flexibility and control over the granularity of the sampling of the solution space.
The concentrated residue was dissolved in 100 μL of methanol and a 1.0 μL sample of the solution was injected into the GC-MS system.
The combination of parallellism and generalized perturbation theory offers the opportunity to perform very exhaustive, fast and accurate sampling of the solution space for the equilibrium cycle reload pattern optimization problem.
A 3.5 μl sample of the solution was applied to glow discharged, lacey carbon film on copper grids (300 mesh).
Each sample of the solution (240 μL) was placed in plates with 96 wells, adding 10 μL of aluminum chloride solution [ 21].
Samples were cooled to room temperature and centrifuged at 5000 rpm for 5 min, and a sample of the solution was analyzed for flavonolignan concentration.
Uniform random sampling of the solution space in any environmental condition is a rapid and scalable way to characterize the structure of the allowed space of metabolic fluxes.
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