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For example, despite some temporal variability, the longitudinal nares samples of Subject A were significantly more similar to each other than to the nares populations of the other subjects (p<0.001 for within-subject to between-subject comparisons; Table 2).
Sequences that were <60 70% similar to HXB2 even in the forward direction were also excluded (this threshold was tuned so that we didn't lose good sequences in the more distant subtype C samples of subject 07; within-patient consensus sequences can be also used with a higher similarity threshold).
For instance, all of the longitudinal groin specimens from Subject A were dominated by corynebacterial species (e.g. C. mucifaciens, C. minutissiumum) that were not present, or much less abundant, in the axilla and nares samples of Subject A, including specimens collected on the same day.
Furthermore, venous blood samples of subject 1 were taken from the antecubital vein, which was kept open with 0.9% saline (100 cl).
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A Swedish blood donor study [5] investigated one of the largest representative samples of subjects and reported photic sneezing in about 24% of the examined subjects.
After genetic counseling and signature of informed consent, peripheral blood samples of subjects were collected.
Different samples of subjects were used for each of these steps.
Further multicenter studies that include larger samples of subjects, especially those of other races, are needed.
Of those remaining, random samples of subjects stratified by pain and psychological distress status were subsequently invited to attend.
The spot urine samples of subjects were diluted with 1 : 50 serum physiologic (0.9% NaCI) for biochemical analysis.
On the other hand, we did study representative samples of subjects from both the community and institutions.
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