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Even with comparatively rich samples of species such as Australopithecus afarensis and A. africanus, most of the specimens are very fragmentary, and even partial skeletons are rare.
However, existing protected areas represent only relatively small, geographically biased samples of species and habitats.
In addition, the potential presence and amount in the biological samples of species that can interfere in the analysis should be evaluated.
In some cases, communities comprised species that are more closely related to each other than expected by chance [2], [4], [7], [8], while in other communities, species are more evenly distributed across the phylogenetic tree [4], [7], [8], [9]; expectations are based on random samples of species selected from a larger regional pool.
First, they can draw on very large samples of species.
However, with the exception of Sibynomorphus, there have been reports of non-molluscan prey being extensively consumed in captivity or found in stomach contents and fecal samples of species from the remaining four genera in Clade E [ 36].
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Because carrion flies are notoriously indiscriminate eaters, they yield DNA from a broad sample of species.
Sampling of species in other categories suggests similarly dire outlooks, with some 24% of reptiles and 32% of crabs thought to be threatened.
Species F is the most distantly related of the sample of species and is known as the "outgroup".
We list five main criteria that well-designed monitoring programmes should meet: representative sampling locations, sufficient sample size, sufficient detections of target species, a representative sample of species, and a sound temporal sampling scheme.
Due to the scope of our analyses, employing experimental methods to determine the rate of false predictions in all or even a representative sample of species is unfeasible.
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