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For determination of complement anaphylatoxin C5a levels in plasma samples of rats, an ELISA-system was developed.
After euthanasia, blood and liver samples of rats were collected for histological and biochemical analyses.
Blood samples of rats were centrifuged at 2,000 g for 10 minutes at 4°C and aliquoted for the respective analytical determinations.
Samples of rats taken from the healed abdominal wall and the healed cecal wall showed integral neo-mesothelial cells layers, with various fibrosis (Figs. 4D and 4E).
Urine samples of rats from all groups were subjected to qualitative analysis of nitrates, pH, protein, glucose, ketone, urobilinogen, and bilirubin.
In short, samples were boiled in ethanol and assayed with a commercial kit (ImmuChem Double Antibody Corticosterone I RIA, MP Biomedicals, CA, USA) intended for the analysis of plasma and extracted urinary samples of rats and mice.
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The 24 h pooled samples of rat urine revealed presence of 6.2% intact prodrug, 7.1% of rhein and 8.9% of boswellic acid indicating their renal excretion.
In order to determine the levels of SCN1A, SCN5A, and SCN8A protein (Nav1.1, Nav1.5, and Nav1.6, respectively), Western blot analysis was performed from samples of rat myocardium at the same developmental stages as PCR analysis had been conducted.
HCl (1 M, 50 µL) and ethyl acetate (1000 µL) were added to samples of rat plasma (100 µL) and diazepam (1 µg/mL, 20 µL) was added as an internal standard.
Samples of rat feces pooled over a period of 24 h showed absence of rhein and presence of 3.1% of intact boswellic acid and 4.6% of boswellic acid emphasizing their intestinal excretion.
The arginase activity obtained from samples of rat liver was used as positive control.
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