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Protein concentrations were determined by spectrophotometry via a BCA protein assay kit, and 20 ug samples of protein were fractionated by electrophoresis in 5% stacking and 15% resolving acrylamide gels and transferred to PVDF filters (Immobilon-P Transfer Membrane).
Samples of protein were harvested following treatment using 2x Laemmli buffer.
Near optimal training can be achieved with only 1000 annotated sites, or roughly three samples of protein sequence alignments.
Control samples of protein alone, substrate alone, or protein and either nucleotide or nucleic acid were also measured; closed-state complexes were not detected in these cases.
For western blot analysis, 20 μg samples of protein lysate were resolved by SDS – polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes (Schleicher and Schuell, Dassel, Germany).
Thus, negative samples of protein coding DNA sequence may also contain TRRs, which could then result in overestimation of the FP.
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Kay, L.E., Xu, G.Y., Singer, A.U., Muhandiram, D.R. & Formankay, J.D. A gradient-enhanced HCCH-TOCSY experiment for recording side-chain proton and carbon-13 correlations in water samples of proteins.
In a laboratory in Boston, Dr. Higgins, a 40-year-old associate professor of microbiology and molecular genetics at Harvard Medical School, oversees graduate students tending samples of proteins drawn from infectious disease cells.
As summarized in [20], until now 16 different PseAAC modes have been used to represent the samples of proteins for predicting their attributes.
In order to detect these effects using samples of protein-coding sequences, we construct and examine three diagnostic statistics.
When compared with the synonymous rates in the same samples of protein-coding genes, lincRNAs showed a stronger correlation (table 5 and fig. 4).
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