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Historically, linkage mapping populations have consisted of large, randomly selected samples of progeny from a given pedigree or cell lines from a panel of radiation hybrids.
The original estimate of the position of Rlnn1[ 3] relied upon data from three small samples of progeny lines (25, 30 and 31 lines), each derived from a different cross.
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Thus, their sample of progeny contains only sib families.
The sampling of progeny and the choice of marker loci were same as in the inbred population.
When the progeny are produced by individual-based pairwise matings such as monogamy and polygyny, the sample of progeny is family-structured.
On the other hand, the sample of progeny from the inbred population consists of families with various degrees of relationship (e.g. cousins).
Simulation with 200 replicates was run for the case of polygyny in the inbred population with Nm = 5 and Nf = 20 parents, n = 100 sample of progeny and L = 15 high-polymorphic marker loci.
The general properties of estimates, e.g. a small bias of estimation from both methods in the noninbred population and a downward bias of in the inbred population, were similar to those observed in Figs 1– 3. A remarkable point in Table 2 is a narrower confidence interval of in a small sample of progeny from a small inbred population.
Our data are unlikely to be affected by biased sampling of progeny within broods (with respect to male order) or variable brood sizes, as there was no correlation between the proportion of the brood sampled and P2 (r = 0.15, d.f. = 21, P = 0.51), or between total or genotyped brood size and P2 (total brood size r = -0.03, d.f. = 21, P = 0.89; genotyped brood size r = 0.03, d.f. = 21, P = 0.91).
A population of recombinant inbred lines (RILs) is a useful tool for mapping QTLs for traits under additive control because RILs represent a permanent sample of progenies for evaluations using replications in different environments.
PCR amplification of genomic DNA samples of T1 progeny using primers specific to bar gene, revealed the presence of transgene in three of the six progeny plants tested (Fig 4B).
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