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Data presented here are the average of the results obtained from three distinct samples of mutant and wild-type plants.
Keeping this in mind, archiving frozen sperm samples of mutant F1 animals, which can be recovered by intracytoplasmic sperm injection (ICSI) [ 33, 34] becomes very attractive.
Neuronal-specific Eno2 (NSE) was detected in serum samples of mutant and WT littermates at postnatal day 4 using the CanAg NSE EIA kit (Fujirebio Diagnostic, Inc., Göteborg, Sweden), according to the manufacturer's instructions.
BFL1 transcript levels in RNA samples of mutant and wild-type were visualized by RT-PCR with BFL1 specific primers (Table 1) using OneStep RT-PCR kit (QIAGEN Inc ,California, USA) reagents according to manufacture's instructions.
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The 3D scatter plot of scores of LDFs illustrates a good separation between the 2- and 12-days old samples of mutants (Fig. 5) which depicts that PC-LDA model is able to differentiate between the early stage of muscle degeneration (2nd day samples) and almost completely degenerated muscles (12th day samples).
Figure 3D shows an SDS-PAGE of unboiled samples of mutants of pmimGFP1.
In principle, this distribution can be determined directly by measuring fitness effects of extremely large samples of mutants (Lind et al. 2010; Hietpas et al. 2011).
To this end, total genomic DNA from four independent samples of mutants 39B3 and 39E7 were Cy3 or Cy5-labelled and cohybridized with wild type DNA labelled with the complementary Cy5 or Cy3 probe on four independent microarray slides.
The plots evaluate all four learning methods and were generated by initially applying tenfold cross-validation (10-fold CV) testing to each of 10 stratified random samples of 50 mutants (25 mutants for stavudine) selected with replacement from their respective inhibitor datasets.
He said other scientists could be given samples of the mutant virus for research only with the permission of Erasmus Medical Center, the National Institutes of Health and virus experts at the Mount Sinai Medical Center in Manhattan.
RbcL was rapidly degraded by subtilisin in samples of the mutant, while the RbcL degradation was clearly delayed in wild-type extracts with bound microcystin (Fig. 6B and C).
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