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Samples of media were collected at 24, 48, 72 and 96 h post-infection and assayed in triplicate for progeny virus by plaque assay using CK cells.
Samples of media were taken at 24 hr intervals and boiled at 97°C for 15 min to denature serum protein.
As protein concentration between different samples of media was identical, loading volume was 30 μl per well for all samples.
A fresh medium containing a test compound was added, and after 72 h, samples of media were collected and centrifuged to remove detached cells.
After 24 h the impeller speed increased to 45 rpm continuously, the lowest speed that held microcarriers in suspension. 1 mL samples of media, containing suspended microcarriers, were removed for cell counting.
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Overall, samples of media-containing guar and jantar composts contained greater amounts of nutrients than the rice hull or wheat straw compost media.
After 24 h, a sample of media was removed for analysis.
Moreover, these results suggest that the common practice of pooling samples of conditioned media prepared over various days may mask functionally critical differences between media batches.
The work demonstrates that, with sufficient care, it is possible to use small representative samples of porous media for use as input to LBM to permit the accurate prediction of the bulk media permeability and filtration performance.
There, the files are compared with samples of the media being measured, using a technology called acoustic matching.
Samples of all media were collected and stored at − 20 °C for analysis of silver concentrations.
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