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Total cellular RNA was extracted from homogenized lysate samples of leukemia cells using the Qiagen RNeasy Plus Mini Kit (catalog no. 74134) (Qiagen, Santa Clarita, CA).
In the present study 12 samples of leukemia and 4 samples of normal mononuclear cells were tested in response to 1266 mechanistically annotated compounds including Food and Drug Administration-approved drugs.
Twelve samples of leukemia (four acute lymphocytic leukemia, four acute myeloid leukemia (AML), four chronic lymphocytic leukemia), as well as peripheral blood mononuclear cells (PBMC) from four healthy donors were used for the compound screen.
Thus, a subset of the NCI60 data (35 samples of five cancer types) was used in the study, including eight samples of renal cancer (RE), six samples of central nervous system cancer (CNS), eight samples of melanoma (ME), seven samples of colon cancer (CO) and six samples of leukemia (LE).
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We quantified expression of 11,873 genes in paired samples of primary leukemia cells and normal leukocytes from 92 patients with acute lymphoblastic leukemia (ALL).
In addition to determining the expression of the WNT7A gene in blood cells, in leukemia-derived cell lines, and in samples of patients with leukemia, the aim of this study was to seek the effect of this gene in proliferation.
Among a sample of predominantly leukemia survivors treated with allogeneic HSCT, smoking within 2 months prior to transplant was an independent risk factor for PTRM.
E. cloacae strain WCHECl-1060 was recovered from a blood sample of a leukemia patient, who was not previously exposed to colistin.
The VSP-A scores were compared with the scores obtained from a sample of childhood leukemia survivors [ 29] and from French controls from a normative sample of 1060 subjects [ 28].
Because both populations were sampled after diagnosis of leukemia among the case population, treatment, past diagnostic procedures, changes in behavior, and changes in chemical exposures over time may all have significant, although immeasurable, impact on the relationships that were explored in our secondary analysis.
In addition to CML BC samples, we used the DSS of different types of leukemia samples in clustering analysis (AML n=10, Ph+ ALL n=3, T-ALL n=3 and B-ALL n=3).
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