After addition of NBD-labeled lipids and incubation to allow probe internalization as usual, samples of labeled macrophages taken at various times were incubated for 5 min with dithionite at room temperature to allow internalization of the reducing agent into the lumen of endocytic vesicles.
Hybridization was carried out using two samples of labeled cDNA (75 pmol of each) that were labeled with Cy3 or Cy5 d-CTP.
where (hat {y}_{i}) and y i are single indexed samples of predicted labels and true labels, respectively.
For the cases of SmCO, MuCO, and CpCO, the GEI features were not stable, and as a result, sometimes samples of these labels were misclassified as other labels.
Features are combined in a classifier that is first trained on a sample of labeled data to learn a prediction model.
We used the single section disector method to estimate the size and number of vesicles contained in a random sample of labeled prefrontal cortico-thalamic boutons (n = 19).
In the "old" days, the company had to ship samples of its labels by mail, a process that took two months; today it can deliver its samples in two weeks.
The appropriate cyanine (Cy3 or Cy5) label (General Electric Healthcare, Piscataway, NJ) was incorporated in samples of cDNA; labeled cDNA was also purified.
The two scientists went to Japan in 1993, visited local markets, and bought many different samples of meat labeled "whale".
Indeed, there were several technical-related difficulties in the measurement of MPs affected centrifugation of samples, labeling of MPs, using HD-FACS methodology and final assay of results obtained.
From left to right are a false-color image from bands 50, 27, and 17, b the ground truth, c training samples using around 10%% of labeled samples per class, d test samples, and e color indexing.
