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Allele sizes are scored by comparison with known control sizes and confirmed by rerunning with additional samples of known size.
Processing involved evaporating samples of known volume to dryness.
The single-grain data from 12 samples of known age (reported by Olley et al. [2004b.
Assay specificity was initially optimized using 103 archived DNA samples of known genotypes.
The sensor was calibrated in an environmental chamber with solid samples of known permittivity over ranges of temperature and humidity.
The accuracy and specificity of the primers was assessed using genomic DNA extracted from meat samples of known sex.
Group-specific amplification reactions and primer sets for SNE were validated with 42 reference samples of known HLA-A alleles.
The reliability of the technique has been confirmed by testing fused silica samples of known thermal conductivity.
Four HIV-1 samples of known subtype, 77 samples with unknown-subtype, and 30 HIV-negative control samples were tested by the new assay.
In order to determine the exact metal content in the prepared nanocomposites, samples of known weight were digested with concentrated HNO3.
Using this method, Gu et al.[14] achieved a yield of between 79 and 109% Hg recovery on soil samples of known Hg concentration.
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