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Molecular weights of samples of isolated poly 3HB) were determined through Gel Permeation Chromatography (GPC) analysis using a Varian GPC50 with an RI detector.
For this, cell samples or samples of isolated poly 3HB) were subjected to methanolysis in presence of hydrosulfuric acid as described previously (Brandl et al.1988, Timm et al.1990).1990
Analysis of the forces exerted by neurons has been limited to theoretical considerations; experimental analysis has been limited to samples of isolated filaments[13] [17]or migrating cells[18], [19].
Nondiluted and 10-fold diluted samples of isolated RNA were analyzed.
RNA was extracted from three samples of isolated bacteriocytes and whole-body samples of adult whiteflies, as described above.
All samples of isolated parasite stages were resuspended in 1 ml of PBS and stored at -80°C until the total RNA was isolated.
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NHRC also sends CDC a sample of isolated virus.
To corroborate this hypothesis, and to differentiate it from hypothesis C, more detailed population studies need to be performed, including sampling of isolated subpopulations with limited migration between them, and thorough sampling of well mixed isolated populations.
These measurements were of connected vesicle widths in the case of the poorly sintered samples and of isolated vesicle radii in the case of the well-sintered samples.
Individual samples of RNA isolated from non-malignant and malignant breast, as well as testis, were purchased from Ambion (Austin, TX).
In each ChIP assay, we compared four independent samples of chromatin isolated from the cortex, hippocampus, and thalamus.
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