Animals were killed by an overdose of sufentanil, propofol and potassium chloride 24 hours after ROSC, and tissue samples of the ischemic and non-ischemic myocardium were collected and then both snap-frozen in liquid nitrogen (stored at −80°C) and fixed in 4% formaldehyde solution followed by embedding into paraffin blocks, respectively.
Next, the samples of the non-ischemic and ischemic treated group were embedded in paraffin wax, sectioned into 5 µm slices and stained with the hematoxin-eosin stain.
In the present study, we used Solexa sequencing technology on small RNA libraries prepared from a mixture sample of rat ischemic cortex at 5 time points (3, 6, 12, 24 and 72 h) of reperfusion after FNS.
In another previous study, IL17 expression was demonstrated in colonic mucosa of CD and ulcerative colitis (UC), while it was not found in colonic mucosa samples of infectious colitis, ischemic colitis, and healthy subjects [ 9].
To explore the potential pathophysiological role of Col5A2 in humans, we analyzed publicly available microarray data acquired from cardiac tissue samples of patients with ischemic cardiomyopathy and controls (Methods).
However, the sample size for subtypes is small compared to genetic study standards, and thus further investigations of this GRS based on larger, well-characterized samples of patients with ischemic stroke are clearly warranted to see if there are subtype-specific associations.
We have previously shown in a propensity score model on the same dataset that patients with reduced FEV1 or hyperinflation on chest radiograph had a lower probability of cTnT sampling, whereas a history of ischemic heart disease, use of aspirin, or radiographic signs of pulmonary congestion increased the probability of cTnT measurement.
The gene expression profiles of ischemic cardiomyopathy samples and the nonfailing controls are kept for binary classification study in this work.
b SERS images of ischemic and control samples at 518 and 736 cm−1.
