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Nonetheless, this species was isolated from a total of 27 samples of host plants, which were not part of the analysis in 2012.
Similarly, representative samples of host snails from the sample wetlands did not demonstrate evidence of Ribeiroia infection (Skelly D, unpublished data).
Examination of individual amphibians as well as representative samples of host snails from the study sites did not reveal evidence that either this parasite or trematode infection in general was playing a role in the causation of limb malformation.
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Additionally, Mycobacterium avium has been isolated from helminths cultured from fecal samples of hosts that were simultaneously co-infected with both helminths and Mycobacterium avium [ 18].
However, replicate sampling of host species on reefs previously targeted in "snapshot" Symbiodinium diversity studies often reveal missed diversity among endosymbiont communities within a host.
We suggest that these findings add support to an interpretation of virus evolution based on extensive sampling of host genomes by itinerant viruses.
Our sample of host individuals was equitably represented across the entire time series (Table 1C).
To maintain a constant, homogenous environment and to eliminate coevolution between phage and hosts, each flask was inoculated with a sample of host cells from a pre-prepared stock.
Rarefaction analyses have often been used to determine species richness curve estimates, given incomplete sampling of species or individuals, but we adapt estimates here to determine whether lineages ('species') were missed at sites, given our sampling of host species.
Phylogenetic analyses coupled with extensive sampling of host and parasites reveal a more complete picture of the complexity of the Escovopsis-cultivar association in colonies of fungus-growing ants, which consists of specialized pathogen species that occasionally switch between distantly-related hosts.
Finally, to examine whether we had exhaustively screened for parasite diversity, we estimated diversity of haemosporidia by constructing rarefaction curves and 95% confidence intervals of parasite species richness using EstimateS v 9.1.0 (Colwell 2013), and extrapolated curves for sampling of host species beyond the 19 species sampled here.
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