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The two non-spiked VS samples (VS2 and VS3) were created by pooling individual incurred CSF samples of established Aβ42 concentration.
Positive controls included normal spleen and breast epithelium for pAMPK and pACC, and breast cancer samples of established ER, HER2 and Ki67.
In order to calibrate and standardise unknown DNA samples, DNA samples of established copy number were included in each PCR in triplicate (ECACC samples, C0075 with a copy number (CN) = 1; C0150 with CN = 2; C0007 with CN = 3; and C0877 with CN = 4).
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However, variations in preanalytical processing of samples and lack of established endogenous controls limit the comparability of results [21].
We measured BPA, eight phthalate metabolites, and PA in the spot urine samples using modifications of established methods [ 24, 25].
63– 65 These high-throughput techniques allowed simultaneously testing of more samples and inclusion of established HCM-causative genes, phenocopy associated genes, and other genes with lesser evidence of pathogenicity.
To identify differentially expressed genes, whose expression might be involved in the formation and maintenance of local recurrence in oral squamous cell carcinoma, we applied global gene expression profiling on primary and recurrent tumor samples of an established orthotopic floor-of-mouth squamous cell carcinoma mouse model [ 8].
Comparisons of samples to establish the statistical significance of difference were determined by the Student t test for independent samples.
Comparisons of samples to establish the statistical significance of difference were determined by the two-tailed Student's t-test for independent samples.
Moreover, the performance of established sampling plans is compared with that of the recently proposed approximation approach with full-ordered observed exponential data.
The strengths of this cross-sectional study were the large random national sample and the use of established scales.
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