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For female mice, duplicate samples of embryonic ovaries were collected at 11.5, 12.5, 14.5, 16.5, and 18.5 days of postcoitum [ 9].
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To further investigate the evolution of the Nups network, we divided the sample of embryonic genes into two groups: The Nups network and the rest of embryonic genes and compared their rate of evolution in each species.
Developmental and morphological studies have been largely performed on human choroid plexus samples of either embryonic or post-mortem origin.
In cultures of seminoma samples, the expression of embryonic markers (PLAP) was retained, and the morphology of seminoma samples was relatively well preserved up to 7 days in hanging drop cultures.
Kidney samples of two different embryonic stages (E14.5 and E18.5) were isolated.
RT-PCR results for samples of all three embryonic ages tested yielded indistinguishable results, indicating that maternal RNAi was effective at reducing zygotic transcripts in embryos at least up to four days AEL.
Sample collection was performed consistently to avoid contamination of embryonic samples with maternal membranes, as this would have jeopardized correct determination of chromosomal inheritance.
Zebrafish samples for qPCR consisted of embryonic and adult hearts and trunk muscle, and were isolated as described above.
To gain insight into MTP expression by the avian embryo, we prepared liver, kidney and small intestine samples from different stages of embryonic development (10, 15 and 20 days), and from 1 and 3 day old chicks, respectively.
This indicates that our samples represent the native development of embryonic towards adult epicardium [18].
The use of embryonic tissue samples were approved by Ethics Committee of the University of British Columbia, Faculty of Medicine.
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