The sampling of diversity is neither random nor representative, and the current list of available genomes reflects particular interests in medically or industrially important pathogens, extremophiles, and organisms with important economic value (e.g., agricultural pests and commensals).
Repeating the analysis for all 26 genomes, including CGH results for the 8 additional LIII genomes, reduced the estimate by over 100 genes to 2,330 ± 5, emphasizing the importance of a balanced sample of diversity for estimating core genome size.
Currently, the dataset with the most extensive sampling of diversity at both var and microsatellite loci within a local population consists of a set of 30 single infection isolates from a local population in Papua New Guinea for which we have sequences for a portion of the var DBLα domain and the alleles for 12 microsatellite loci (Barry et al., 2007; Chen et al., 2011).
We believe that as more target sequences become available, our predicted false negative rates will tend to increase for a given published signature both as a result of better sampling of diversity and as a result of failure to detect newly evolved variants.
A core collection (N = 21) was also used to test the association between the identified polymorphisms and traits, and this collection contains a representative sample of diversity in cultivated Vitis vinifera L. and in different Vitis species and genera [ 67] (Additional file 10).
If put in the right context, these unidentified sequences may represent valuable samples of fungal diversity and ecology.
Thus they were based on a small sample of taxonomic diversity and diversity within the cystatin superfamily.
It has been suggested that increasing the sampling of rodent diversity [ 2] and/or mammalian diversity [ 18] would have supported rodent monophyly.
Twenty participants were purposively sampled, for diversity of opinion, from the population of non-menopausal married women over age 20, living in Shah Faisal Colony, Karachi.
Here, we sought to better understand how diet alters rumen microbial diversity, using a shotgun sequencing approach that allowed deep sampling of microbial diversity across multiple individuals.
