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Sixty samples of cocoa products were provided by the Canadian Food Inspection Agency or purchased in local stores.
Analysis of 32 samples of cocoa powder (16 alkalized and 16 natural) for OTA showed an incidence of 100%, with concentrations ranging from 0.25 to 7.8 ng g−1; six samples exceeded 2 ng g−1, the previously considered European limit for cocoa.
Analysis of 32 samples of cocoa powder (16 alkalized and 16 natural) for OTA showed an incidence of 100%, with concentrations ranging from 0.25 to 7.8 ng g−1; in six samples the OTA level exceeded 2 ng g−1, the previously considered European Union limit for cocoa.
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Sampling of cocoa beans was done between December 2014 and February 2015.
Ripe cocoa bean samples were taken from each farm, as well as a sample of cocoa beans that had been fermented and dried in their shells, ready for export.
For the particular sample of cocoa butter used in this study, the fatty acid composition as determined by gas chromatography (GC) is shown in Table 1, together with the fraction of total double bonds represented by each component.
Samples consisted of cocoa, dark chocolate, milk chocolate and NIST SRM [2384] Baking Chocolate having certified values for -catechin and -epicatechin.
The method was applied to the analysis of samples of Brazilian cocoa beans, and only one sample was found to have a PAH content above the maximum limit defined by the European Union legislation.
Figure 2 Sample chromatogram of cocoa bean extract.
Figure 1 provides an example chromatogram showing the separation of the various epicatechin and catechin sterioisomers while Figure 2 provides a chromatogram showing the stereoisomer separation on sample of roasted cocoa beans.
Samples of fermented dried cocoa beans (2 kg) were thoroughly grounded into fine powder and homogenized.
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