Sentence examples for samples of buffer from inspiring English sources

In addition a series of eight samples of buffer only (PBS) were analyzed as blanks.

Briefly, 300 µL samples of buffer were collected at the beginning and end of relaxation protocols in experiments with ACh or CPA (corresponding to AA/BH4 incubation times of 30 and 60 min).

To 2 mL samples of buffers containing various levels of Pi, formulated as described above, 40 μL of 0.5 M 90CE dissolved in acetol was added to give a final 90CE concentration of 10 mM.

A 500 μl sample of buffer alone was also included as a control, as well as an appropriate volume (500 μl per reaction) of freshly made substrate (nitro phenyl phosphate 0.0742 g/10 ml 0.1 M sodium acetate pH 3.6).

Samples of assay buffer, biotin-conjugated detector antibodies were added to microwells and the plates were incubated at room temperature for 2 h.

DNA was isolated from the gel slice using a Qiagen MinElute kit (Valencia, CA, USA) and 6 sample volumes of buffer QG.

Following the addition of sample buffer, samples were boiled for 3 min and separated by SDS-PAGE (8% acrylamide).

The raw data was normalized and transformed into a Excel spreadsheet, and the percent inhibition (PI) ratio was calculated using the following formula: PI = 1-{(OD of sample - OD of buffer)/(OD of negative control – OD of buffer)} X 100.

The samples of insulin and buffer components from Sigma (USA) were used without additional purification.

For each assay, 490 μl of sample buffer consisting of 0.5%% (w/v) of starch in Tris HCl (50 mM, pH 7.0) was incubated at the desired temperature.

Briefly, 9μL of sample buffer, 9μL of reaction buffer and 1μL of enzyme mix were added to the punches with thermo cycling conditions according to the manufacturer's instructions.