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Equal protein amounts of all (patient or donor) samples of appropriate protein fractions were pooled and 1 μg or 0.75 μg of total protein amounts were used for 1D western blot analyses for vinculin or fermitin-3, respectively.
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2 In addition to the challenges associated with sampling of appropriate SF specimens, the reliability of polarising microscopy is poor.
Repeated micro-biologic sampling of appropriate body fluids such as blood, sputum, cerebrospinal fluid, and urine may help to diagnose and treat a possible infection early.
One tube 22, condition 2 was excluded because several analytical parameters showed aberrations that exceeded 3 standardized residuals, and we concluded that this tube did not contain a sample of appropriate quality.
If, on the other hand, the purpose is to describe (fine-scale) spatial variation, or variation as function of environmental or population differences, samples of the appropriate spatial scale or relevant environments or populations should be sequenced.
The resulting suspensions were serially diluted in the same diluent and 1 or 0.1 mL samples of the appropriate dilutions were poured or spread on nonselective and selective agar plates.
Protein samples after addition of appropriate sample buffer were separated either on 10% SDS-PAGE gels [ 55] or on 3-10% non-denaturing PAGE gels as described previously in Drykova et al. [ 38].
In this study, we tested a PCS anxiety scale to determine the measure's psychometric properties across to different samples of age-appropriate men.
Therefore, there is an interest in using whole genome amplified (WGA) DNA samples created from patient samples instead of native DNA samples to increase the availability of appropriate samples to query the performance of the assays.
The Lausanne branch of the Ludwig Institute for Cancer Research (LICR-LB) performed extensive pre-testing of donor samples and selection of appropriate donors.
To determine if each of the entities obtained by the NER system was actually of the correct classification, a sample of the appropriate size (Table 1) was randomly taken from the extracted entities of each of the two categories.
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