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Although histological characterization clearly demonstrated inflammatory processes at work in samples of advanced DN, RMA based array and gene ontology (GO) analysis could identify only limited regulation of GO categories associated with inflammation suggesting a more sensitive approach was needed [4].
This feature is also noted in clinical samples of advanced prostate cancer (40).
Similarly, another study used immunohistochemistry and immunofluorescence to show that POSTN was localized to stromal fibroblasts in human samples of advanced gastric cancer (Kikuchi et al., 2014).
Significantly, mir-200c∼141 cluster is specifically silenced in clinical samples of advanced prostate cancers and highly metastatic prostate cancer PC-3 (Porkka et al, 2007).
Among the analyzed 26 advanced OCCC tissues, 14 (54 %) samples of advanced OCCCs showed scored as HIN-1 weak staining (0 or +1 immuno-reactivity), and 12 (46 %) were HIN-1 strong staining (+2 or +3 immuno-reactivity).
In 1996, it had already been demonstrated that CD49d expression in CLL is variable, with higher expression of CLL samples of advanced (Rai III, IV) than early stages [ 89].
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Although the next-generation sequencing technologies provide a vast amount of data samples for advancing genomic research, the ever-increasing volume of genomic data has become a tremendous challenge on multiple fronts (Durbin, 2009; Fritz et al., 2011; Kozanitis et al., 2010).
In this sample of mainly advanced patients, the emotional dimension of HRQL had deteriorated for two years after the start of first generation PI-ART, and the subjective perception of adverse effects made a major contribution to this decrease.
In a cross sectional multicentre study carried out on a sample of 240 advanced cancer patients with pain, background pain and BP-EP intensity in the last 24 hours were measured using both a 6-point VRS and a 0-10 NRS.
Less inclusive approaches are opinion surveys of population samples, however, advanced forms of opinion surveys seek to elicit preferences around the attributes that can influence acceptance or rejection of a policy proposal.
MMPs are key molecules of tumor cell invasion, including EOCs [ 42] and, since the majority of samples were advanced stage EOCs, MMP19 could be a new player in the dissemination of these tumors and experiments are now ongoing to test its presence and role in advanced stage EOCs.
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