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The analysis of non-defined environmental samples may require a species specific reverse primer to ensure only Methanospirillum hungatei JF1 is detected.
These samples may require further study to know whether they have an interspecific background.
We expect that larger samples may require up to 2 3 d.
The best analysis of tumour samples may require a combination of proteomic, genetic, epigenetic and functional characterisation.
With the turkey model, the engraftment window of 7 9 days, and some patient samples may require extended times, which are not possible in this rapid model.
Such unbalanced samples may require the introduction of a third λ parameter class of branches to accommodate (i) over-sampled within-species branches, (ii) within-species branches and (iii) among-species branches.
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In addition, achieving a sufficiently large replication sample may require a meta-analysis of many smaller studies with an accompanying decrease in power due to population heterogeneity in sample make-up and phenotyping methods.
However, low levels of the pest can be difficult to identify in soil samples, and may require multiple years of sampling before the pest population reaches a detectable level.
The method still needs to be tested in a wide variety of clinical and food samples and may require the use of protease inhibitors and affinity chromatography to partially purify and concentrate the toxin.
OM-S-treated sputa were successful in both protocols; if the Xpert buffer is not added during the H.2 procedure, sample viscosity may require repeat testing.
They may not be suited to the quantification of environmental chemicals in the sample, which may require different collection equipment as well as different SOPs.
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