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In the case of short time history samples loading spectra have to be extrapolated.
When the furnace is pumped for the first time after samples loading, short cycles between 500 and 1800 °C at fast heating rates (≈ 400 °C·min− 1) are sufficient to achieve very low blanks.
Samples were reconstituted in 30 μl of samples loading buffer (20 mM citric acid, 2% (v/v) acetonitrile and 0.1% (v/v) formic acid).
To determine the quantitative expression levels of the transcripts, samples loading was monitored and normalized by the expression of β-actin transcripts.
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PC2, encapsulating 9% of the total variation modelled, further distinguished samples according to their variety: all Apo samples loaded negatively on PC2, all IR64 samples loaded positively.
The signal intensity of histone H3 was constant among all samples loaded.
Cycle sequencing was performed using Applied Biosystems BigDye V 3.1 and samples loaded on the ABI-3730 capillary system.
Pulse EPR measurements were performed on 25 30 μL samples loaded into 2 mm i.d.
For the LDA cards the RNA samples loaded were derived from 3 donors.
The amounts of samples loaded were monitored by the detection of β-actin (Fig. 1B).
The sample loaded with 8.5% curcumin is the second candidate.
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