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For immunoblot analysis of the samples listed above, proteins were transferred to nitrocellulose membranes using the Bio-Rad Mini Trans-Blot electrophoretic transfer cell.
pombe genome (for doped samples listed above), or to the S288c reference 58 for all other strains, using BWA version 0.7.3 (Li and Durbin, 2009).
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Equal amounts of total RNA from each sample listed above were mixed for cDNA library construction.
Control seeds were sampled at the same inoculation time listed above and considered as uninoculated.
Many of the methods listed above were established for samples of 1 to 10 g and have to be modified when working with sample sizes of < 1 g.
The open-data initiatives listed above are only a small sample of the overall efforts in the United States.
One approach that can minimize the problems listed above is to use large sample sizes comprised of subjects from comparatively homogeneous ethnic backgrounds for each study population [15].
All concentrations listed above are postmixing.
The seasonings listed above are just recommendations.
The dailies listed above are all free.
The cells were then treated with the samples list above plus LPS (1 μg/mL) or LPS alone for 20 h.
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