Author(s): Priscilla Chan, The New York Times Learning Network Bridget Anderson, The Bank Street College of Education in New York City Suggested Time Allowance: 1 hour Objectives: Students will: 1. Propose a sample life cycle of a plastic soda bottle.
To support gene discovery and gene model verification in the genome of the wheat leaf rust fungus, Puccinia triticina (Pt), we have generated Expressed Sequence Tags (ESTs) by sampling several life cycle stages.
Two biological replicates were generated and sequenced for mixed samples of five life cycle stages.
RT-qPCR was carried out on cDNA samples from various life cycle stages of S. parasitica to obtain a detailed expression profile of SpSsp1 (Fig 3).
Individual samples from the life cycle stages of the bumblebee differed in the expression of a wide array of genes, including genes involved in amino acid storage, metabolism, immunity and olfaction.
TobEA is a map of gene expression from multiple tissues sampled throughout the life cycle of the tobacco plant and is intended to be used as a reference data set for plant researchers.
Thus, the nCounter® platform and our custom P. falciparum probe set could be a valuable screening method to assess RNA quality in crude lysate samples and determine parasite life cycle stages, both of which are useful to identify meaningful groups of samples for more costly and labor intensive experiments, such as RNAseq.
With microarray data of 33 tissue samples covering the entire life cycle of rice, fairly high OsCESA gene expression and rather variable OsCSL expression were observed.
Then, we focused on an integrative analysis of co-expression profiling and regulations using 33 tissue samples from the entire life cycle of two rice varieties.
The data in this study was gathered from samples for the six life cycle stages: merozoite, ring, trophozoite, schizont, gametocyte and sporozoite.
