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These include lack of an overall sampling design, biases in the collection of isolates from animal samples, non-compliance of state departments of public health with NARMS protocols, lack of such basic data as genotype information on the organisms isolated, and, for animal samples, lack of measurements of bacterial load.
A Mantel-Cox logrank test was used to compare survival curves after determining that assumptions of the test were met (e.g., independent, random samples; lack of correlation among covariates).
Other limitations noted during the literature review include the overuse of clinical samples, lack of longitudinal data, appropriate comparison groups, large samples, and ethnic group analysis.
The reasons for this may include; lack of biological knowledge due to limited access to tumour samples, lack of DIPG preclinical models, tumour heterogeneity and poor drug delivery [35].
Studies suffer from small numbers, selected samples, lack of clarity regarding the intervention delivered and in the method of assessment, the outcome measures chosen, and the length of follow-up.
For OTA, these can be due to an insufficient number of samples, lack of representative sampling of all relevant foods, or difficulties in obtaining representative long-term food consumption estimates.
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Four samples lacking of follow-up HPV DNA were excluded from final analysis.
Since development of infection was a rare event in our sample, lack of significance could be related to low power.
These limited numbers of prior studies showed mixed results and were hampered by either small sample, lack of quantification of LVSI, or lack of a validation set.
This study is severely limited by small sample, lack of control, no details of recruitment, unvalidated and subjective outcome measures and poor reporting of acupressure.
The strengths of this study are: a well-examined cohort sample, lack of age and gender bias and a good quality of longitudinal follow-up.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com