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As the vast majority of studies investigated BD type 1 (BD1) samples, it remains to be established, however, whether this clinical delineation of EO is present also in BD type 2 (BD2) patients.
While Shotgun Protein Sequencing was shown to work well for low-complexity samples it remains unclear how it will scale up for complex samples.
First, although the virus genome sequences were found in the arthropod samples, it remains uncertain that these sequences represent active viral infections of these animals.
Although the data presented here show that the panel outperforms individual biomarkers in tissue-based samples, it remains to be seen if this is the case also in noninvasive material.
While we know that a 2 h recovery period is sufficient to significantly increase stress and strain values in stress-relaxed samples, it remains to be established how quickly a measurable recovery effect occurs and if the failure properties are completely recoverable given sufficient time.
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Although, we failed to capture a substantial amount of population diversity through the present sampling, it remains unlikely that sampling artefacts alone can account for similar intraspecific divergences found among freshwater and marine species.
We accept that while snowballing may not lead to a genuinely representative sample, it remains the most pragmatic and cost-effective method for case-detection in low resource settings [22].
Third, the study used a representative sample; it remains unclear whether results would persist in clinical samples.
Thus we think that even if the ancestral sequence built here is biologically more relevant than previously calculated ones (because of better taxonomic sampling), it remains quite uncertain.
Third, considering that the surveyed Hungarian facilities were not part of a random sample, it remains not clear whether participating LTCFs (24 %) are representative of the whole reference population.
On the one hand, notwithstanding our extensive sampling, it remains the only instance from across the species range where we found coexistence of salamanders of two different mtDNA lineages.
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CEO of Professional Science Editing for Scientists @ prosciediting.com