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The precise reason for the absence of the body fluid specific markers in these known samples is unclear.
Most of the analytical approaches identified have been applied to samples of a few hundreds or thousands of observations, and their performance in more limited samples is unclear.
Cannabis and to a lesser extent tobacco use are more stigmatised than alcohol use in Australian society, with the 2010 National Drug Strategy Household Survey estimating that 45% of Australians approved of regular alcohol use, compared with 15% for tobacco and 8% for cannabis [ 4], p157, but how such cultural effects relate to the differences in response across samples is unclear.
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In addition, the exact fluid fraction of their recovered samples was unclear.
With case 29 the labeling of the pre- and post-treatment samples was unclear.
The mechanism(s) involved in lead's impact on genomic DNA methylation levels in cord samples are unclear.
PCA seemed to be inappropriate for such a large data set (212 samples × 31 compounds) because the resulting classification of the samples was unclear and chaotic.
Supplementary Figure S3A shows the distributions of the (AATGG) repeat, but the difference between SCA31 and the other samples was unclear because the (AATGG) repeat is enriched in human centromeres (Grady et al., 1992).
Three articles, however, reported on patients from multiple European countries [ 39, 52, 57], while the geographical location of the study population or samples was unclear in one article [ 54].
Whether they were overlooked or are exceedingly rare and thus not sampled is unclear.
Fourth, our analysis relied on a small sample size, and the representativeness of our final sample is unclear.
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