The next day transfer of high molecular weight samples was ensured at 450 mA for one hour.
Quality of the samples was ensured in a Bioanalyzer 2100.
Additionally, a structural equality by chance concerning both sub-samples is ensured.
The crystalline nature of the sample was ensured by X-ray diffraction measurement after each annealing.
Due to the irregular mycotoxins distribution a proper sampling was ensured according the EU requirements [35].
The diversity of the patient sample was ensured for age (45 60 years, n = 29; 61 80 years, n = 38; >80 years, n = 14), gender (59 women), professional activities (yes, n = 34; retired, n = 57), and place of living (55 urural6 rural).
The diversity of the care providers' sample was ensured for age (<45 years, n = 11), gender (8 women), specialty (11 GPs, 6 rheumatologists, 4 orthopedic surgeons, 8 [4 GPs] delivering alternative medicine), and place of practice (23 urural6 rural).
Each sample was ensured to have at least 30 million reads and, on average, there were 48 million reads per sample.
Correction for transfection efficiency of each sample was ensured by normalization of the firefly luciferase values to renilla luciferase that was co-transfected into the system (Fig. 2).
A large variability in the biological and sociodemographic features of the sample was ensured to study the general performance of the questionnaire.
Random sampling was ensured by advancing the cutting stage a random distance (between 1 and 50 μm), before sections were collected (Coggeshall and Lekan 1996; Howard and Reed 1998) (Fig. 1a).
