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Quantitation of changes in protein abundance of large number of samples is challenging and requires automatic processing means.
Increasing the number of targets studied in small tissue samples is challenging but newer technologies may help reach this goal.
Nevertheless, working with clinical samples is challenging.
However, experimental availability of EH samples is challenging.
In contrast, obtaining high-quality RNA from a significant number of brain samples is challenging.
As the majority of clinical specimens are formalin-fixed paraffin-embedded (FFPE) tissue, the subsequent analysis of DNA extracted from such FFPE tumour samples is challenging.
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Translating this approach for laboratories that analyze clinical samples is challenged by quality issues with human tissue RNA.
The selective study of 4q-located D4Z4 repeats in human samples is challenged by the presence of D4Z4-like sequences on almost all human acrocentric chromosomes (Lyle et al., 1995; Winokur et al., 1994).
The analysis and determination of N-nitrosamines (NAs) in water samples are challenging and demanding.
Meanwhile, indeterminate samples are challenging for clinicians and researchers.
It is well established that FFPE tumour DNA samples are challenging to amplify and sequence due to damage resulting from the fixation process and due to sample heterogeneity.
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