Nonetheless, the process of generating simulative samples introduced the noisy data, which decrease the classification accuracy especially for positive samples.
Furthermore, the ability to apply molecular quantification techniques to FFPE samples introduces the possibility of conducting large retrospective validation studies for new molecular diagnostics on well annotated samples with long-term follow-up.
For normal tissue, the problem of heterogeneity might be negligible, but in cancer, that is well known for its heterogeneous set-up, applying different tissue samples introduces the risk that the heterogeneity is behind the expression differences, and not the degradation.
Direct VEGF staining is only performed on a small tissue sample, introducing the possibility of sampling error given the heterogeneity in angiogenesis distribution commonly seen in tumours.
For this reason comparative culture or freezing studies can be performed only on a pool of samples, introducing individual variation into the experiment.
The CSF-based QC samples introduced into the method are stable under conditions expected during normal assay execution, and CSF samples can be stored at −70°C for at least 3 months.
However, there was some receptiveness to the idea of at least debating how repeated lapses should be penalised and there was enthusiasm across the sample for introducing the notion of individual responsibility for ensuring best practice hand hygiene.
Regarding the new samples introduced in this study, the Anatolian Greek component was approved by the IRB of Aristotle University, Thessaloniki, Greece.
Both approaches were applied to identical areal samples of 66 GBM, which are a subset of the samples introduced in Section 2.3.
We suggest that the ancestral germplasm of P. abyssinicum was a small sample, introducing a bottleneck into the diversity of this gene pool that still survives today.
