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The PL efficiencies of samples in solution were estimated using a method previously reported [14].
All these techniques have the advantage of requiring samples in solution, which greatly facilitates control over homogeneity and allows quick calibrations.
Highly desirable qualities for a new technique would therefore have to include the multielemental capability of ICP-AES and similar or lower detection limits to GFAAS, in addition to being designed for the analysis of samples in solution.
As shown in Fig. 4, this protocol contains three alternative sub-protocols that are each optimized for low-complexity samples in solution, protein gel bands, or high-complexity samples.
PL quantum yields (QYs) were determined by comparison of the integrated fluorescence intensity of the QD samples in solution with that of standard of known QYs (Rhodamine 101 (R101) ethanol solution (0.01% HCl, QY = 100%) as the standard).
The room-temperature PL quantum yields (QYs) of nanocrystals (NCs) were determined by comparing the integrated emissions of the NC samples in solution with those of a fluorescent dye (rhodamine 6G in ethanol or rhodamine 101 in 0.01% HCl ethanol solution) with identical optical density.
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Ageing of the membrane was mimicked by immersing samples in solutions containing sodium hypochlorite and commercial oxidants with various concentrations, temperatures and soaking times defined by experimental designs.
The PM was deposited, through dipping on the sample in solution, on the structured Si surfaces.
The biologically active conformation may be a single conformation or a small subset from the conformations sampled in solution or a new conformation, induced by protein binding.
With increasing sophistication in solution-based and ensemble-oriented biophysical approaches such as electron paramagnetic resonance (EPR) spectroscopy, atomic resolution structural information can be directly linked to conformational sampling in solution.
This could be due to a physical breakup of the sample in solution; however, a corresponding Si release was not observed at this point in time.
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