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Interference from the spectra of solvent can be avoided by measuring the samples in gas phase, thereby extending the application range of DAOSD and relevant approaches.
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NBO-aided electronic structure analyses performed for the eight conformers representing minima in the potential energy surface of -S-anabasine indicate that both steric and electrostatic factors are determinant in the conformational distribution of the sample in gas phase.
The organic phase was separated and concentrated before injection of the totality of the sample in gas chromatography (GC, Thermo Finnigan Trace GC; HP-5 Agilent column).
The present study indicates that the total dose-rate and areal dose-rate at a fixed illumination area are equivalent descriptors for the achievable TEM image resolution for samples immersed in gas environments.
In summary, the present work shows that in situ observations by TEM of samples immersed in gas environments must be performed at low dose-rates in order to maintain resolution possibly due to suppression of electric charging in both the gas and the sample.
Breath samples are collected in gas sampling bags, and samples are passed through the trap at a flow rate of about 50 cm3/min.
When examined using a high-resolution instrument and with the samples in the gas phase, the individual normal-mode absorption lines of polyatomic molecules will be separated into a series of closely spaced sharp lines.
This provides a clear indication of the crucial role of sample charging on the observed resolution reduction at high electron-dose-rates for samples immersed in gases.
SPV transients for both types of samples in different gases show anomalous spikes of SPV during both 'light-on' and 'light-off' events (Figure 2).
Solid-state precursors are sampled in the gas line via quartz crystal monitors and injected into the O2 microwave plasma with pulse time durations of a few seconds.
The original batch test has been modified to include solid-phase microextraction (SPME) fibers for sampling in the gas phase.
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