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Cells were suspended at a density of 4 × 105 cells/mL, and distributed into 96-well microtiter plates (100 μL per well) already containing 100 μL of two-fold serial dilutions of rhu IL-2 or samples, in complete medium.
To measure glycosaminoglycan (GAG) and DNA content in the NP and AF, samples in Ambion® KDalert™ lysis buffer solution were homogenized in a tube rotator O/N at 4 °C, whereas samples in Complete Lysis-M EDTA-free buffer were homogenized in a TissueLyser II (Qiagen) for 2 × 30 s at 20 Hz.
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The Val-9Ala genotypes determined for the blinded QC samples were in complete agreement with the genotypes determined for the study samples.
*For core singleton ALSPAC sample not in complete case sample, n=10 949.
Among these cases, the detected HPV types in oral and urinary samples were in complete agreement in 8 cases (44.4%), and were consistently similar in 16 cases (88.9%).
The results of the MB assay for these samples were in complete agreement with the results obtained by the conventional nested PCR [ 8] and the reported TaqMan assay [ 7].
The NMR spectra of our synthetic sample were in complete agreement with the reported data.
After the first buffer change, there should be ∼0.3 mM DDGly remaining in the sample if complete equilibration occurs.
Therefore it is not possible to add new samples in a completed study.
First, participant samples were maintained in complete anonymity during GenoChip analysis.
First, only 23%% of eligible phone numbers in the sample resulted in completed survey interviews, and only 49%% of those successfully contacted for the survey chose to participate.
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