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One of the other major cellulolytic bacteria usually found in the rumen samples, Ruminococcus flavefaciens, was observed to be more in the solid and liquid fraction of the rumen samples in animals fed with dry roughage compared to green roughage (Table 3).
When alveolar hemorrhage was considered alone, however, there was a significant difference between ventral and dorsal samples in animals placed in the supine position.
All samples in animals treated with 2 mg/Kg were infected, and no animals treated with 10 mg/kg of cefazolin had any bacteria detectible on their bone or hardware.
Moreover, the levels of protein, electrolytes, enzymes, and other components in seminal plasma samples in animals such as brown bears [ 11], rabbits [ 12], stallion [ 13], and bactrian camels [ 14] have been investigated, but the useful information was very limited in these studies.
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Mathematical models can be used to quantify irreversibly binding tracers, but they would require an input from arterial plasma [31], which, as discussed above, is far more readily sampled in animals larger than mice.
For each species (sheep and goat), the stratum sample sizes determination was carried out using the formula by Bennett et al. [ 21] implemented within the ProMESA software program for statistical sampling in animal populations [ 22].
The second group of clinical samples consisted of 199 blood samples (in some animals a sero-sanguineous fluid from the thoracic cavity was taken), each from one animal.
Finally, analysis of urine samples in two animals demonstrated unchanged renal clearance of 4.2 ± 1%.
The initial temperature was used for correction of both samples in all animals.
As expected, the proportion of clean samples was higher in animals sampled alive (55%) than after death (4%; p < 0.001), while the proportion of moderately to severely hemolytic samples was similar in hunted animals (77%) and animals found dead (70%).
Samples collected in animal study 1 and animal study 2 were prepared and analyzed in November 2009 and May 2010, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com