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Eleven genes that showed expression differences between the two fox samples, in analysis of transcriptome data, were selected for validation by RT-qPCR.
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For each analysis, only those samples in that analysis were used in the filtering process.
The analysis included both related and unrelated mutation carriers in order to maximise the number of samples in the analysis.
Information on specimen age was unavailable for 4 samples in the analysis.
Yi people in Yunnan Province are represented by two samples in the analysis: Samei (Yi-2) and Luoluobo (Yi-3).
The FFPET and frozen samples in our analysis were stored for varying periods of time prior to RNA extraction.
The number of samples in each analysis is shown in the figures.
After quality control we included 1832 DNA samples in our analysis.
This was because of the need for homogenous samples in proteomic analysis.
We followed these same cluster classifications for the samples in this analysis.
We detected a total of 142 genera from the 13 samples in this analysis.
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