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First, using an "on-strip" processing method, we found that protein can be isolated from exudate samples in amounts compatible with large-scale mass spectrometry-based proteomic analysis.
After 24 h the cells were properly washed with DPBS (de-ionized phosphate buffer saline) and incubated with samples in amounts of 4, 8, and 16 mg in triplicates and maintained at 37 °C for 24 h under 5%% CO2 humidified environment along with control.
In a therapeutic context, P. aeruginosa can be present for extended periods of time, and HQNO can be detected in 80% of CF sputum samples in amounts correlating with the P. aeruginosa cell density at the onset of pulmonary exacerbation (49).
By computational analysis of alternative splicing based on exon-level microarray data, we show that common types of solid cancers (including breast, cervical, colorectal, lung, and prostate cancers) exhibit large variation among samples in amounts of aberrant skipping and inclusion that are non-randomly associated with the expression levels of splicing factors.
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For example, increasing cement from 5to10%0% in sandy soil samples increases shear resistance by 30% and, in relation with that, increasing crushed glass in stabilized sand samples in amount 10, 30, 50% increases shear resistance in order about 70, 98, 244%.
Panduratin A compound, and the standards; Ascorbic acid and Quercetin were dissolved in DMSO and sampled in amounts of 10 μL of 1 μg/mL along with 10 μL of 1 μg/mL silymarin and added into 300 μL of the reagent TPTZ separately in triplicate using DMSO as blank.
Other elements present in both samples in lesser amounts are Al, Ca, O, Mn, Cl and Si.
The merits of this protocol include low cost, easy operation, high sensitivity and acceptable stability, which make it ideal for the detection of environmental samples in trace amounts.
A range of effective consolidation stress values can be attained by first equilibrating shale samples in varying amounts of suction, to vary the water content.
Cyanobacteria were the most abundant phylum in the POF sample but were detected in all samples in considerable amounts (Fig. 4a).
There is much less spruce in the violin than maple, and we were unable to acquire samples in sufficient amounts for this set of analyses.
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