Study design: Samples identified as anti-HBc positive (389/4359, 8.9%) during an 11-month period were investigated for HBsAg, anti-HBs, anti-HCV and anti-HIV.
In a common tier based approach, samples identified as ADA screen positive are confirmed for the binding specificity of the antibodies to the drug molecule via a confirmatory assay.
The samples identified as unclassifiable are assigned a "no-class" assignment and it is shown that these samples have a much higher error rate than samples assigned to a class.
PCR reactions were repeated independently, three times, for the two samples identified as mating type a (see Results).
For instance, samples identified as subtype 3a, were correctly classified as such based on four different possible hybridization signal outcomes, as described in the results.
Due to some scale differences in the responses between samples, identified as differences in spread of M-values between the arrays, scale normalization between the arrays was performed.
Samples identified as subtype 2b displayed one of the following three combinations: signal for the U2bx probe only, signals for U2bx + NS2b or signals for U2By + NS2b.
Samples identified as subtype 3a displayed one of the following four combinations: signal for U3a1 only, signals for U3a1 + NS3ax, signals for U3a1 + NS3ay or signals for U3a1 + NS3ax + NS3ay (table 1).
Since none of the cord blood samples identified as containing MSP1 had evidence of malaria parasites (blood smear and PCR negative) at delivery, we postulated that soluble MSP1 crosses transplacentally from maternal to fetal circulation during gestation.
The mean tumor content of samples identified as being mutant by dideoxy sequencing was 78% (range 35 95%) and 71% (range 35 95%) in the case of pyrosequencing (Fig. 2A).
BLAST analysis failed on samples identified as T. palustris in the phylogenetic analysis, either because of lack of resolution (rbcL) or because the species was not present in the online records (trnL-F).
