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Samples have usually been from one center and highly selective, and as few as 10% of the patients in each ICU received the diary intervention [ 2, 8, 15, 24].
Electron microscopy has sufficient resolution, but the samples have usually been prepared with aldehyde fixation, solvent dehydration, plastic embedment, and heavy-metal staining, which can all introduce artifacts to native structure.
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Of samples that quantitated with <10 copies (5 μl -1 in a final quantitation assay of all our DNA extracts, 39/41 had previously produced unambiguous sequence data, while samples that did not amplify in this reaction (68 samples) had usually failed to produce sequence data in previous positive PCRs (though 8 had previously produced successful sequences).
This lack of specificity could be bypassed on the basis of the age of the animal at the time of sampling, as PoNoV infections have usually been found in older animals [ 31] or by usage in serie of a pan-calicivirus primer pair for confirmation, as the SR80/JV33 primer pair [ 32].
Genome-wide association studies have usually used samples of unrelated cases and unrelated controls to evaluate evidence of associations and obtain relative risk (RR) estimates.
Comprehensively sampled studies of cave organisms have usually focused on species delimitation and cryptic diversity.
Previous studies on revision after shoulder arthroplasty have usually included small samples, and they have rarely compared survival between groups.
Geneticists interested in the events that have shaped human populations over the last 50,000 years or so have usually preferred to sample individuals living in the same location as their ancestors (indigenous people), often excluding individuals whose grandparents do not all come from the same local area, or whose ancestors are known to have migrated during historical times [13].
Gradual discontinuation interventions are much more effective, with variable cessation rates, but such trials have usually had small sample sizes.
Previous studies of negative symptoms have usually involved smaller patient samples that were recruited to a research project.
Since sampling frequency has usually been low (mostly monthly sampling intervals) in the acidic mining lakes investigated, a switch in the life cycle of Cephalodella to sexual reproduction and the production of resting eggs might have been missed.
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