Exact(3)
Forty six out of the 48 (96%) samples had consistent interpretative results on retesting (Table 2).
Benign samples showed hypomethylation across the entire HES5 promoter, whereas matched tumour samples had consistent hypermethylation across all 60 CpGs assayed (Fig. 2a, b and Supplementary Figure 4, see section on supplementary data given at the end of this article).
The genomic region, which covered the CHEK2 locus and where all CHEK2 1100delC samples had consistent calls, as defined by CGH regions analysis, spanned three successive BAC clones, almost 2 kb, and five genes.
Similar(57)
The strong points of the present study were the use of a population-based sample, having consistent socioeconomic information at three points in life and not having detected studies with similar design and analysis in the literature.
Only samples from donors that had consistent results in all four performed experiments were finally selected for distribution to the participating centers.
They have had consistent sampling over much of the Australian EEZ and extensive taxonomic study by a currently active scientific group.
Penetrance of aberrant chromosomal areas across the genome was demonstrated using Aberration Detection Method 2 (Agilent Genomic Workbench Lite Edition 6.5.0.18, Agilent Technologies), a quality-weighted interval score algorithm that identifies aberrant intervals in samples that have consistent gain or loss log ratios based on their statistical score.
"They haven't had consistent history.
Similar analysis for the H3K9me3 ChIP-seq assay revealed that the 23 °C-generations samples had a modest but consistent reduction of H3K9me3 levels for NHGs in both the wild-type and the hrde- 1 mutant animals when compared with the 15C-G3 sample (Additional file 5).
In this study, the effect of amplification differences between plates was not enough to cause genotyping inaccuracy, as duplicate sample pairs consistently had genetic distances below our threshold of 0.2 and our positive control sample had a consistent genotype in all runs.
The histopathological results of the tissue samples had been found to be consistent with ABC.
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