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Measured samples for the same time frame were gathered from six International Monitoring System stations in the Southern Hemisphere with distances to the source ranging between 680 (Melbourne) and about 17,000 km (Tristan da Cunha).
Biological replicate samples for the same time point were summarized by their median expression profile, and the summarized expression profile for each RefSeq gene was centred to have median zero.
The same cut-offs were applied in Limma after considering Tempranillo skin and flesh samples for the same time point as replicates to identify transcripts with consistent oscillation in expression along the pericarp.
In this case, Tempranillo skin and flesh samples for the same time point were considered replicates to pick out transcripts with consistent expression along Tempranillo pericarp that was the tissue analysed in Verdejo.
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In terms of genetic composition, the translocated fish were not significantly different from either sample of randomly-selected fish, but FST was near 0.0 for the samples from the same time period, and near 0.01 for the samples from different time periods.
Studying samples from the same time course for both gene expression and protein abundance lead to identification of potential targets for plant proteins directly manipulated by the pathogen.
Matching control replicates that have not been subject to light induction were sampled for the same time length albeit more sparsely for the Q-PCR and in vitro assay data.
The percentage of total products observed at different inhibitor concentrations was divided by the percentage of products observed for protein samples preincubated for the same time with dimethyl sulfoxide.
To exclude artificial changes in expression due to different culture periods, we compared the mRNA and miRNA expression profiles of PBMCs in matched irradiated and non-irradiated samples incubated for the same time periods.
kern-0pt} {V_{i,with,out,inhibitor} }}} right) times,100where V i with inhibitor is the initial velocity observed at different inhibitor concentrations and V i without inhibitor is the velocity observed for enzyme samples pre-incubated for the same time with inhibitor free in DMSO.
Control albumin samples were incubated for the same time without the addition of glucose.
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