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Eight samples were randomly selected from 34 positive samples for sequence analysis of NPM1 mutation.
This study confirms the high potential of massively parallel pyrosequencing in the scanning of DNA samples for sequence variations.
Screening large numbers of target regions in multiple DNA samples for sequence variation is an important application of next-generation sequencing but an efficient method to enrich the samples in parallel has yet to be reported.
Within a single sequencing experiment, it has the capacity to analyze the exonic regions of hundreds of genes in tens of samples for sequence variation using as little as 1 μg of input DNA.
Within a single experiment, this method can sequence the exonic regions of hundreds of genes in tens of samples for sequence and structural variation using as little as 1 μg of input DNA per sample.
Consequently, various approaches to specifically enrich target genomic regions simultaneously thereby allowing barcoding of samples for sequence multiplexing have been developed and can be divided into polymerase chain reaction- (PCR-) based and non-PCR-based strategies.
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JF prepared DNA samples for sequencing.
Armed with these prescriptions, we submitted our samples for sequencing (for me, that involved convincing the kind folks at the local LabCorp to draw blood for a test they'd never seen before).
The manual preparation of samples for sequencing is a very work demanding process.
Samples for sequencing were taken from those alive or dead after bioassays.
Mature worms with a visible clitelum (0.4∼0.6 g/worm) were used for preparing cDNA samples for sequencing and cRNA probes for array hybridization.
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