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The applicability of the recommended procedure was estimated on madder root, waste and river water samples for separation and measurement of the four anthraquinone dyes.
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Six healthy subjects were chosen to collect the blood sample for separation of lymphocytes and collection of squamous epithelial cell by scraping.
HPLC with ultraviolet detection has been used for the analysis of AZM in bulk samples, for the separation of related compounds produced during synthesis and for acid degradation studies.
Blood samples for serum separation were collected from the jugular vein of apparently healthy camels using labeled plain vacutainer tubes following proper physical restraining.
Processing of samples for rapid separation of extra- and intracellular fluids was performed by using silicone oil centrifugation with perchloric acid in the bottom layer (Rottenberg, 1979).
The sodium salt of BiEDTA has been described as a novel solute forming self-generating density gradient during ultracentrifugation of serum samples for the separation of lipoproteins [ 24].
Samples of bovine NS and tissue extracts from NS, nasal mucosa, small intestine, liver, kidney and bone were selected at random from the available samples for the separation of AP isozymes.
Blood samples for the separation of plasma for M30 and M65 ELISA analyses were collected from all eligible subjects entered into the Cancer Research UK-sponsored phase I Trial of OXi4503, where the drug was administered by 3 × weekly intravenous infusions to patients with advanced solid tumours (CR-UK Protocol Number: PH1/098 and Eudra CT Number: 2004-002375).
This variability increases the sample needed for separation at 12 months.
The supernatant protein was quantified in duplicate using Bradford Ultra (Expedeon, Cambridgeshire, UK) and prepared in Laemmli sample buffer for separation using a 12% SDS-PAGE at 75 µg protein/sample and subsequently transferred onto a nitrocellulose membrane (Sigma).
GFP tagged proteins were then isolated using GFP-TRAP beads (ChromoTek) according to the manufacturer's instructions and the immunoprecipitated proteins were subsequently resuspended in Laemmli sample buffer for separation by SDS-PAGE.
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