Surveys were performed 3 4 days prior to sampling for rats, during rat sampling and then repeated for 3 4 days after rat trapping.
The miRNA array data and the array definition have been submitted to Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/) with accession number GSE66134 for human samples and GSE66195 for rat samples.
Separately, the dark and light samples for each rat were then pooled and volume measured.
Graphene-based electrodes have been used to monitor DA in biological samples, for example rat striatum [ 82], mouse hippocampus tissues [ 83], and human serum and human urine [ 84, 85].They have also been used for simultaneous determination of DA, AA, and UA [ 90].
For samples from rat liver, CD-COBS Charles River rats were anesthetized with Nembutal and sacrificed.
Serum levels of CRT were determined by ELISA following the manufacturer's instructions (USCNK, Life Science Inc, Wuhan, China; catalogue number E91486Hu, for human samples; catalogue number CSB-E14943r for rat samples).
Four to six breasts of each rat were milked, and the contents were pooled to a single sample for each rat.
After an additional 10 min, a blood sample for each rat was collected for determination of alanine aminotransferease (ALT).
For plasma samples from rats following the 3 mg/kg dose, the elution of metabolites for negative ion mass spectrometry was achieved at room temperature on a Thermo Syncronis HILIC, 5 µm, 150 × 4.6 mm column equilibrated with 10%% solvent A (ammonium acetate 10 mM) and 90%% solvent B (acetonitrile) at a flow rate of 1.0 mL/min.
We thank Dr. Urmila P. Kodavanti, National Risk Management Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA for brain samples from rats that were exposed to diesel engine exhaust.
