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Even with developmentally matched samples, for primary cell cultures, culture conditions and days in vitro (DIV) also affect the dynamics of gene expression as shown in [ 34].
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Treated sample for primary transcript sequencing and untreated sample for processed transcript sequencing were analysed with the Agilent Bioanalyzer system as described above.
The degree of representativeness of the GEMCAS sample for primary care has been reported in more detail by Moebus et al. [ 11].
Mouse and rat embryonic samples used for primary cultures were developmentally matched based on the protocol provided by Charles River Laboratories [ 48, 49].
A small portion of the sample used for primary culture (or a blood sample or DNA derived from the donor) should be frozen or processed immediately.
All 48 participants provided data for the reactogenicity assessments and blood samples for the primary immunogenicity analyses at day 28.
JP and MB prepared samples for the primary screen.
Swab and fine needle aspirate (FNA) samples for the primary cultivation were collected from clinically confirmed Buruli ulcer patients in the Mapé Basin of Cameroon.
However, the areas did not differ on the key variable we were attempting to predict (intention) (p=0.084) so we combined the two samples for the primary analysis.
Samples for biotinylated primary antibody staining were further blocked for endogenous biotin using streptavidin solution (Vector Laboratories) for 15 min followed by incubation with 0.01% (w/v) biotin (Sigma)/PBS.
Our results suggest that 10% was the best entire sampling ratio for primary core collection of apricots.
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