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If RNA has been degraded, ribosomal RNA bands appear fuzzy or smeary; do not use such samples for preparing messenger RNA.
Patients referred for lung cancer diagnosis and/or staging by means of EBUS-TBNA were enrolled, the adequacy of the obtained samples for preparing cell blocks was assessed, and the additional pathologic or genetic information provided from cell block analysis was examined.
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The measurement equipment and the procedures for preparing samples for determination of selected radionuclides are presented next.
Only RNAs with an OD260 OD280 ratio >1.80 and no discernible degradation were used for preparing samples for sequencing of mRNA.
We thank Kimberly Cook and Ruth Johnson at the Mayo Clinic for preparing the samples.
We thank Tatjana Knyazeva for preparing cDNA samples.
For samples prepared for SEM the aldehyde fixation was followed by repeated osmification according to the OTOTO protocol [ 24].
Slides were stained for cytokeratin and LSC analysis performed as for samples prepared by IMS.
Samples were prepared for laboratory and for in situ experiments.
Human samples previously prepared for pachytene analysis were labeled for SCP3, centromeres and SUMO-2/3.
Wet stool samples were prepared for examination by light microscopy for the presence of helminths.
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