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Samples for nuclear magnetic resonance (NMR) measurements contained 4 mM cantheicidin-BF in TFE-d3/H2O (9∶1, v/v) at pH 6.5, or in 300 mM SDS-d25 at pH 6.5.
Samples for nuclear counts, FACS, RT-PCR, and Westerns were taken at indicated times.
Samples for nuclear magnetic resonance (NMR) analysis consisted of ~1 mM peptide in 50 mM sodium phosphate, 90%H2O/10%% H2O at pH 5.5 or 7.0.
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Finally, 4',6-diamidino-2-phenylindole (DAPI) antifade reagent (Invitrogen, Carlsbad, CA, USA) was added to each sample for nuclear staining.
Finally, 4',6-diamidino-2-phenylindole (DAPI) antifade reagent (Invitrogen) was added to each sample for nuclear staining.
Sampling for nuclear magnetic resonance (NMR) spectroscopy was performed at the same time than sampling for OD600 for growth and samples taken were stored at −80°C until NMR analysis.
Liver samples collected for nuclear extract preparation (3 animals/time-point) were processed immediately after the surgery without freezing.
However, assignment of estimated nuclear DNA contents to specific C-values in the present study is presumptive in that no karyological investigations were conducted on the algal samples used for nuclear DNA content estimates.
Assignment of estimated nuclear DNA contents to specific C-values in the present study is presumptive in that no karyological investigations were conducted on the algal samples used for nuclear DNA content estimates.
Measurements of the isotopic ratios of the elements uranium and plutonium in small amounts, typical of those found in environmental samples, are required for nuclear safeguards and security, for the control of environmental contamination and for the detection of nuclear proliferation.
77% of the patients whose primary tumor samples negatively stained for nuclear p65, showed no recurrence up to 8 years post radical prostatectomy.
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