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This method was followed to prepare all samples for metal analysis by flame AAS.
Previous studies suggested various methods for digesting different samples for metal analysis (Nabil 2010).
Water samples for metal analyses were collected into 100 ml plastic bottles and nitric acid (HNO3) added to the samples to bring the pH down to 2 for the metals to remain in solution.
Samples for metal analyses were digested with concentrated HNO3 in a microwave digestion system.
Blood samples for metal analysis were drawn into trace-metal free trace-metal free
In a preliminary study, Gonzales analyzed house dust samples for metal contaminants, including cadmium, nickel, copper, tin, lead, and silver.
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Nitric acid was used to preserve samples for metals analysis.
Samples for metals were preserved by adding 3 mL of concentrated HNO3.
The blood sample for metal analysis was collected from a cubital vein in a 6-mL plastic vacutainer containing potassium EDTA as the anticoagulant (BD Medical).
– fill in a lifestyle and a diet questionnaire, – donate a 15 ml blood sample (for molecular tests), – provide a 50 ml urine sample (for metal internal dose assessment), – provide a lock of hair cut next to the root in the occipital area of the head (for metal internal dose assessment).
At recruitment, each study subject is asked to: v – fill in a lifestyle and a diet questionnaire, – donate a 15 ml blood sample (for molecular tests), – provide a 50 ml urine sample (for metal internal dose assessment), – provide a lock of hair cut next to the root in the occipital area of the head (for metal internal dose assessment).
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