To clarify and expand the biological significance of the Q-profile on a larger scale we employed 2D-DIGE on select pairs of amniotic fluid samples matched for maternal age, parity, gestational age at amniocentesis and membrane status.
Associations with GDM across pyrosequenced loci were modeled using linear mixed models with a random intercept for sample, adjusting for maternal age, pre-pregnancy BMI, infant sex, maternal smoking, and self-reported ethnicity.
Sample sizes for maternal inbreeding coefficients were 435 unique mothers for birth date, 407 for birth weight, 381 for first year survival, 388 for summer survival and 365 for winter survival (16 mothers had all offspring die during their first summer).
For negative controls, the maternal blood was used as a mock sample for a maternal heterozygous, fetus heterozygous genotype.
For IgM measurements, plasma samples from maternal blood were diluted 1 200 and those of cord blood 1 50.
Figure 1 illustrates the relationship between quartiles of cumulative lead measures and mean Alu and LINE-1 methylation levels in cord samples after controlling for maternal age, education, smoking during pregnancy, and child sex.
Study participants were compared with non-participants using independent samples T-Tests for maternal age at infant's birth, and Chi-square tests for independence for relationship status at infant's birth, highest level of education attained, country of birth and initial feeding intention.
Samples of maternal grandsons were not available for grandsire G01.
The panel of specific IgE antibodies varies by blood sample: egg white, milk, and peanut for children's samples; birch or oak, ragweed, and Timothy grass for maternal samples; and Dermatophagoides farinae, Dermatophagoides pteronyssinus, dog epithelium, cat dander/epithelium, German cockroach, mouse urine protein, and Alternaria alternata measured for all samples.
