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The analyzing pathologist, blinded to study group, scored the samples for lung injury (see Additional file 1) [ 20].
There was a 5-fold range in the potency of the samples for lung injury, and samples from high-emitting vehicles (both diesel and gasoline powered) had the highest pulmonary toxicity per unit of mass.
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Armed with a powerful GRPS, the researcher can then go to an independent sample (e.g., a general population sample) – there is no need to enrich the sample for lung cancer or smoking above that found in the general community.
Twelve hours after oral administration there was little in other tissue samples except for lung and liver.
Normal white blood cells were employed as controls for the tumor samples, except for Lung 4 and Lung 5 in Table 1 where normal lung tissue served as controls.
Another limitation of our study was that sample size for lung cancer subgroups was small.
Based on statistical analysis of the quantified images, the microendoscope's limit of detection was determined to be 10 CFU in the case of skin samples and 10 CFU for lung samples.
For light microscopy, samples of lung tissue were embedded in paraffin, cut into 4 μm slices, and stained with hematoxylin and eosin.
We tested samples of lung and spleen for influenza A virus by a slightly modified version of a previously described real-time reverse transcription PCR (RT-PCR) that targeted the matrix gene (7 ) and for phocine and canine distemper virus by 1-step RT-PCR using previously described primers that targeted the P (phosphoprotein) gene (8 ).
For the random sputum samples of lung cancer patients, the detection rate was 47.5%, but in the induced sputum, BAL and pleural fluids, the detection rate was up to over 70.0%.
Lymphoid tissues and samples of lung tissue were cultured for mycobacteria and scored (total number of culture-positive samples) as described in [ 22].
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